NASC

NASC ID: N850321

Name: WiscDsLox289_292O15

ABRC stock number: CS850321

Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line.

Associated Polymorphisms:

Wiscseq_DsLox289_292O15.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G60408 and AT5G60410.1-P355 (chr 5 pos 24293710 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG797664.

Wiscseq_DsLox289_292O15.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G28007.1-P355 and AT3G28010.1 (chr 3 pos 10411590 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank ID: KG797663.

Wiscseq_DsLox289_292O15.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G48620.1 (at chr 5 pos 19719471 (C/19719471-19719517) on the TAIR10). The sequences' 1-47 bps mapped on the genome, 86-100 bps onto the T-DNA. Pools P 1, R 2, C 6 N 3. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO453005.

Donation Date: 2004-12-01

Donated by:

University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman

Donor Number: WiscDsLox289_292O15

Part of : Wisconsin DsLox T-DNA lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-2

Transformation method: Agrobacterium

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT3G28007 AT3G28010 AT5G48620 AT5G60408 AT5G60410